論文・著書:2008-9
95. M. Ihara*, T.
Suzuki*, N. Kobayashi, J. Goto and H. Ueda "Open-sandwich
enzyme immunoassay for one-step noncompetitive detection of
corticosteroid 11-deoxycortisol" Anal.
Chem.
81,
8298-8304
(2009).[DOI][Abstract] * equal
contributions
92.
T.
Sakata, M. Ihara, I. Makino, Y. Miyahara and H. Ueda "Open
sandwich-based immuno-transistor for label-free and
non-competitive detection of low molecular weight
antigen" Anal.
Chem.
81(18), 7532-7537
(2009).[DOI][Abstract]
91.
H.
Iwanaga, H. Tsuzuki, Y. Kamiyama, and H. Ueda "Verification
of specific reaction between airborne antigen and
immobilized antibody at gas-solid interface"
Anal.
Sci.
25 (9), 1101-1106
(2009).[Abstract]
* Selected as a Hot Article
90.
R.
Iwasaki, H. Kiuchi, M. Ihara, T. Mori, M. Kawakami and H.
Ueda "Trans-splicing
as a novel method to rapidly produce antibody fusion
proteins" Biochem.
Biophys. Res. Commun.
384(3), 316-321
(2009).[Abstract]
89. T. Zako, H. Nagata, N. Terada, A. Utsumi, M. Sakono, M.
Yohda, H. Ueda, K. Soga and M. Maeda "Cyclic RGD
Peptide-Labeled Upconversion Nanophosphors for Tumor
Cell-Targeted Imaging" Biochem.
Biophys. Res. Commun.
381, 54-58
(2009).[Abstract]
88.
W.
Liu, M. Kawahara, H. Ueda, T. Nagamune "The influence of
domain structures on the signal transduction of chimeric
receptors derived from the erythropoietin
receptor"
J.
Biochem.
145, 575-584
(2009).[Abstract]
87. T.
Dojima, T. Nishina, T. Kato, H. Ueda and E.Y. Park
"Comparison of the efficiencies of different affinity
tags in the purification of a recombinant secretory
protein expressed in silkworm larval hemolymph "
Biotechnol.
Bioproc. Eng.
14,
281-287
(2009).[Abstract]
86.
K.
Tanaka, M. Kawahara, H. Ueda and T. Nagamune "Selection and
growth regulation of genetically modified cells with
hapten-specific antibody/receptor tyrosine kinase
chimera" Biotechnol.
Prog.
25, 1138-1145
(2009).[DOI]
85.
J.
Dong, M. Ihara and H. Ueda "Antibody Fab display system
that can perform open sandwich ELISA" Anal.
Biochem.
386, 36-44
(2009). [Abstract]
84.
T.
Sogo, M. Kawahara, H. Ueda, M. Otsu, M. Onodera, H.
Nakauchi and T. Nagamune "T cell growth control using
hapten-specific antibody/interleukin-2 receptor
chimera" Cytokine
46, 127-136
(2009). [Abstract]
83. E.Y. Park, M. Ishikiriyama, T. Nishina, T. Kato, H.
Yagi, K. Kato and H. Ueda "Human IgG1 Expression in
Silkworm Larval Hemolymph Using BmNPV Bacmids and its
N-Linked Glycan Structure" J.
Biotechnol.
139, 108-114
(2009). [Abstract]
82. M. Ishikiriyama, T. Nishina, T. Kato, H. Ueda and E.Y.
Park "Human Single-chain Antibody Expression in the
Hemolymph and Fat Body of Silkworm Larvae and Pupae Using
BmNPV Bacmids" J. Biosci.
Bioeng.
107,
67-72
(2009). [Abstract]
81. M. Sakono, T. Zako, H. Ueda, M. Yohda and M. Maeda
"Formation of highly toxic soluble amyloid beta oligomers
by the molecular chaperone prefoldin" FEBS
J.
275, 5982-5993
(2008).
[Abstract]
80.
Y.
Lee, T. Asami, I. Yamaguchi, H. Ueda and Y. Suzuki "A new
gibberellin detection system in living cells based on VH/VL
interaction of antibody" Biochem.
Biophys. Res. Commun.
376, 134-138
(2008). [Abstract]
79.
K.
Yoshitake, S. Waki, and H. Ueda "Dimerization-based
homogeneous fluorosensor proteins for the detection of
specific dsDNA" Biosens.
Bioelectron.,
23, 1266-1271
(2008) [Abstract]
78.
W.
Liu, M. Kawahara, H. Ueda, and T. Nagamune, "Construction
of a fluorescein-responsive chimeric receptor with strict
ligand dependency" Biotechnol.
Bioeng.
101, 975-984
(2008)[Abstract]
77.
Kawahara, M.,
Shimo, Y., Sogo, T., Hitomi, A., Ueda, H. and Nagamune, T.
"Antigen-mediated migration of murine pro-B Ba/F3 cells via
an antibody/receptor chimera" J.
Biotechnol.
133,
154-161
(2008) [Abstract]
76.
T.
Sogo, M. Kawahara, K. Tsumoto, I. Kumagai, H. Ueda and T.
Nagamune "Selective expansion of genetically modified T
cells using an antibody/interleukin-2 receptor
chimera" J. Immunol.
Methods
337, 16-23
(2008) [Abstract]
75. S. Takeda,
S. Tsukiji, H. Ueda, and T. Nagamune "Covalent split
protein fragment-DNA hybrids generated through
N-terminus-specific modification of proteins by
oligonucleotides" Org. Biomol.
Chem.
6, 2187-2194
(2008) [Abstract]
総説・著書
伊原
正喜,上田 宏
"第三の免疫測定法「オープンサンドイッチ法」の開発"
化学工学
72(9)
486-488
(2008)
上田 宏
"抗体の二本鎖構造を生かした低分子の非競合的検出法の開発” 化学と生物
46(4) 252-258
(2008) [Abstract]
招待講演
上田 宏,"新規免疫測定原理とマイクロ化学の融合による次世代バイオセンシング"
次世代マイクロ化学チップコンソーシアム研究会,東京大学(12/8,
2009)
上田 宏,"オープンサンドイッチ法による低分子免疫測定技術の開発と各種バイオ計測への応用"
酵素工学研究会第62回講演会,東京大学山上会館
(11/13,
2009)
H. Ueda "Novel methods to produce fusion proteins in
mammalian cells: fusions in DNA, RNA and protein
levels", APBioChEC 2009, Kobe
International Conference Center (11/25,
2009)
H. Ueda
et
al. "Artificial
modification of cellular activity through antibody-receptor
chimera",
Seminar
at National Chiao
Tung University, Hsinchu, Taiwan (11/6,
2009)
H. Ueda "Open
sandwich immunoassay as a sensitive analytical method of
various biomolecules", Seminar at National Chi Nan
University, Puli, Taiwan (11/5, 2009)
H. Ueda "Open
sandwich immunoassay as a sensitive analytical method of
various biomolecules", Seminar at Academia Sinica, Taipei,
Taiwan (11/2, 2009)
H. Ueda
"Open sandwich
immunoassay for the realtime imaging of intracellular
protein phosphorylation",
The
International Symposium on the MEXT Priority Research
Project 'Cell Proliferation Control',
"Cell
Cycle and Cell Architecture", Nagoya
(2/28,
2009)
H. Ueda
"Open sandwich
immunoassay as a general approach for the sensitive
detection of small molecules",
Seminar
at Applied
Chemistry Department, NCTU, Hsinchu, Taiwan (12/22,
2008)
上田 宏
"新原理の免疫測定とナノテクの融合による新しい生体認識分子工学をめざして" CNBI定例セミナー,東京大学工学部11号館講堂
(12/17,
2008)
Hiroshi Ueda, Hiroto Iwai,
Bengu Ozturk and Masaki Ihara "Open Sandwich
Selection to Improve the Affinity of Anti-Peptide Antibody
Using Unconjugated Osteocalcin Peptide" 4th Korea-Japan
Workshop on Combinatorial Bioengineering, Kookmin
University, Seoul, Korea (11/22, 2008)
上田
宏
"蛋白質工学による新しい検査原理・診断用分子の構築"
第26回医用高分子研究会講座「検査・診断を支える高分子」,産業総合研究所臨海副都心センター
(11/11,
2008)
上田 宏
"何でもバイオセンサーを目指した融合蛋白質デザイン"
生体機能関連シンポジウム 若手フォーラム,東京工業大学(すずかけ台,9/17,
2008)
上田
宏 "抗体融合タンパク質を用いた低分子センサーの創出" 第8回日本蛋白質科学会年会,東京(6/13,
2008)
H. Ueda "Open sandwich
ELISA for sensitive detection of various
biomolecules",
Antibody
Engineering Asia, Singapore (2/27,
2008).